Search results for "COPIE DE GENE"

showing 2 items of 2 documents

Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example

2008

ABSTRACT We investigated the effects of internal primer-template mismatches on the efficiency of PCR amplification using the 16S rRNA gene as the model template DNA. We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up to 1,000-fold of the gene copy number, depending on the primer and position of the mismatch.

Base Pair MismatchGene DosageBiologyPolymerase Chain ReactionApplied Microbiology and BiotechnologyPrimer extensionlaw.inventionDNA POLYMERASElawRNA Ribosomal 16SMethodsCopy-number variationGenePolymerase chain reactionDNA PrimersADN CIBLE[SDV.EE]Life Sciences [q-bio]/Ecology environmentGeneticsEcologyRibosomal RNA16S ribosomal RNACOPIE DE GENEReal-time polymerase chain reactionPseudomonas aeruginosaPrimer (molecular biology)Food ScienceBiotechnologyApplied and Environmental Microbiology
researchProduct

Indicateurs pour l'évaluation de l'impact de produits phytosanitaires sur la composante microbienne de la qualité biologique des sols

2011

National audience

[SDE] Environmental SciencesCYCLE DU SOUFRECYCLE DE L'AZOTENOMBRE DE COPIE DE GENES FONCTIONNELS[SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering[SDV]Life Sciences [q-bio]DEGRADATION DES PESTICIDESBIOINDICATEURSACTIVITES ENZYMATIQUES[SDV.IDA] Life Sciences [q-bio]/Food engineeringSTRUCTURE DE COMMUNAUTES MICROBIENNESABONDANCE DE COMMUNAUTES MICROBIENNES[SDV] Life Sciences [q-bio]POUVOIR DE COLONISATION DE CHAMPIGNONS MA[SDV.IDA]Life Sciences [q-bio]/Food engineering[SDE]Environmental SciencesDIVERSITE BIOCHIMIQUE[SPI.GPROC]Engineering Sciences [physics]/Chemical and Process EngineeringTEST DE GERMINATION DE SPORESComputingMilieux_MISCELLANEOUS
researchProduct